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SRX1012754: GSM1669149: JC2581_Xenograft_MCF7_PR_E2+PG r4; Homo sapiens; ChIP-Seq
3 ILLUMINA (Illumina HiSeq 2000) runs: 16.8M spots, 839.9M bases, 527.4Mb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: ChIP-seq of ER and PR in xenografted MCF7 tumors grown in mice [xenograft]
PRJNA282506 • SRP057757 • All experiments • All runs
show Abstracthide Abstract
Exploring effect of estrogen and/or progesterone treatment on xenografts tumors in vivo Overall design: MCF7 cells implanted in mice (two each), subjected to different treatment. Six replicates of each condition we ChIPed.
Sample: JC2581_Xenograft_MCF7_PR_E2+PG r4
SAMN03570728 • SRS924531 • All experiments • All runs
Organism: Homo sapiens
Library:
Instrument: Illumina HiSeq 2000
Strategy: ChIP-Seq
Source: GENOMIC
Selection: ChIP
Layout: SINGLE
Construction protocol: immunoprecipitated DNA was end-repaired, A-tailed, ligated to the sequencing adapters, amplified by 18 cycles of PCR and size selected (200-300 bp) followed by single end 4 sequencing on an Illumina HiSeq 2000 according to the manufacturer’s recommendationas described in Chandra, T. et al. Independence of Repressive Histone Marks and ChromatinCompaction during Senescent Heterochromatic Layer Formation. Mol Cell 47, 203–214 (2012)
Experiment attributes:
GEO Accession: GSM1669149
Links:
External link:
Runs: 3 runs, 16.8M spots, 839.9M bases, 527.4Mb
Run# of Spots# of BasesSizePublished
SRR20009774,373,816218.7M139.1Mb2015-05-22
SRR20009786,121,517306.1M192.7Mb2015-05-22
SRR20009796,302,297315.1M195.6Mb2015-05-22

ID:
1467606

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